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Methods: The small intestinal (jejunum and ileum) with the vascular pedicle was isolated from rats and the superior mesenteric artery and the portal vein were cannulated for vascular perfusion. Krebs Ringer solution (KRS, 37ºC) was perfused at 1 ml/min and MABP was measured continuously by means of a pressure transducer connected to the arterial inflow line. After organ stability, RBC suspension (10% hematocrit in KRS) was perfused at the same rate, and the MABP was monitored. Then the preparation was washed with KRS and subsequently perfused with HlyA- or ProHlyA-RBC. In each condition a sample of the solution leaving from portal vein was taken at 2-min intervals and ATP was immediately measured by a luminescence technique. The same protocol was performed in the presence of 100 µM suramin (a non-selective purinergic receptor blocker). Results: i. perfusion of HlyA-RBC increased MABP value (205 ± 77 % n=7 p<0.05) compared with RBC, while the values measured with HlyA-RBC + suramin, and Pro HlyA-RBC were not different of that obtained with RBC. Consistent with these results, the highest ATP values were measured in the samples from Hyla-RBC in a range of 4.41 to 54.07 n=3) compared with those measured with only RBC (ATP values in a range of 0.09 to 1.18 µM n=3). Conclusion: Perfusion of Hlya-RBC suspension induces intravascular ATP release, which activates purinergic receptors that mediate contraction of the mesenteric blood vessels. Thus, uropathogenic E. coli infections could have a relevant impact on arterial blood pressure by changes on peripheral resistance lingerieshop | Scopri di più lingerieshop | Scopri di più su lingerieshop | Scopri di più qui
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