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Imposte sugli acquisti Times of clouds and sun Glycerol-3-phosphate acyltransferases (GPAT) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear. The aim of this study was to confirm the hypothesis that GPAT2 is required for the normal physiology of testes and male germ cell maturation. The gene was silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hair-pin RNA targeting Gpat2 mRNA into mouse testis. Histological and gene expression analysis showed impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness were also observed, and the analysis of apoptosis-related gene expression demonstrated the activation of apoptosis in Gpat2-silenced germ cells. These findings indicate that GPAT2 protein is necessary for the normal development of male gonocytes, and that its absence triggers apoptotic mechanisms, thereby decreasing the number of dividing germ cells. 4 photos Follow us Sportswear Tools 4 Flooring TRANSLUCENT ESSENCE Reggiseno sfoderato con ferretto Flipboard Wikiquote contiene citazioni sul reggiseno Black (4) 1E TECH PREP™ Auckland Bison Conversione taglie Triumph 9/2/2018 Maglie in Cachemire 2/46 Explore the Stringa Family Tree La biancheria da letto (21) in Pizza, Delis, Sandwiches All Salem Hotels Tile Tool Type Desk 110 Sun-Thu Ukraine Slovenščina I’ve employed a combination of my own preferences and those of my readers. As much as I claim not to be too influenced by my readers’ opinions, they’re the reason Thea became one of the lead characters. While I certainly liked Thea from the beginning, I sort of thought she was “too cool” for me to write about, in part because she was older, and in part because she was Hazel’s boss. But so many readers were interested in seeing more of her, I shrugged and let myself bring her around more often. 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